Stress On The Central Role Of Nucleic Acids And Proteins In Living Cells: Biological Oxidation And Intermediary Metabolism Of Carbohydrates, Lipids, And Proteins, And The General Properties Of Enzymes And Enzyme Catalyzed Reactions In The Intact Cell And Cell-Free Systems. Laboratory Work Stresses Use Of Modern Techniques Used In Biochemical Analysis And In EnzymeAssays. Selected Experiments To Demonstrate The Dynamic Aspects Of Biochemistry In Living Cells And In Cell-Free Systems.Prerequisite: BIO 166 And BIO 167 And BIO 238 And One BIO CourseAt 200 Level Or Above (Not BIO 230) And CHE 234 And CHE 235.

Molecular aspects of cellular function; properties of biomolecules, their biosynthesis and breakdown; structure and function of proteins and enzymes, metabolites, membranes and nucleic acids; cellular mechanisms of energy transduction; integration and control of cell metabolism. Experiments cover a variety of modern techniques in biological chemistry.

This course adopts an interdisciplinary approach to understanding the fundamentals of biochemistry, including an introduction to the basic concepts and most common research methods. The focus will be upon the major macromolecules and chemical properties of the living systems. The goal is the development of an integrated appreciation of how biomolecules act and interact to catalyze reactions, to synthesize and degrade biomolecules, and utilize or release energy. Both catabolic and biosynthetic pathways will be discussed. The biochemistry underlying the conversion of information contained in DNA to cellular macromolecules through replication, transcription and translation will be covered. Primary topics include the structure, function, and metabolism of amino acids, proteins, carbohydrates, nucleic acids and lipids; the physical properties of water, pH, buffers and enzyme kinetics; DNA replication and gene regulation.